The spread of LAGLIDADG homing endonuclease genes in rDNA
نویسندگان
چکیده
منابع مشابه
The spread of LAGLIDADG homing endonuclease genes in rDNA.
Group I introns that encode homing endonuclease genes (HEGs) are highly invasive genetic elements. Their movement into a homologous position in an intron-less allele is termed homing. Although the mechanism of homing is well understood, the evolutionary relationship between HEGs and their intron partners remains unclear. Here we have focused on the largest family of HEGs (encoding the protein m...
متن کاملThe LAGLIDADG Homing Endonuclease Family
The LAGLIDADG protein family includes the first identified and biochemically characterized intron-encoded proteins (Dujon 1980; Lazowska et al. 1980; Jacquier and Dujon 1985), as described in this volume by Dujon. It has been variously termed the ‘DOD’, ‘dodecapeptide’, ‘dodecamer’, and ‘decapeptide’ endonuclease family, based on the conservation of a ten-residue sequence motif (Dujon 1989; Duj...
متن کاملLAHEDES: the LAGLIDADG homing endonuclease database and engineering server
LAGLIDADG homing endonucleases (LHEs) are DNA cleaving enzymes, also termed 'meganucleases' that are employed as gene-targeting reagents. This use of LHEs requires that their DNA specificity be altered to match sequences in genomic targets. The choice of the most appropriate LHE to target a particular gene is facilitated by the growing number of such enzymes with well-characterized activities a...
متن کاملThe evolution of homing endonuclease genes and group I introns in nuclear rDNA.
Group I introns are autonomous genetic elements that can catalyze their own excision from pre-RNA. Understanding how group I introns move in nuclear ribosomal (r)DNA remains an important question in evolutionary biology. Two models are invoked to explain group I intron movement. The first is termed homing and results from the action of an intron-encoded homing endonuclease that recognizes and c...
متن کاملCharacterization of Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease, reveals a unique mode of DNA-binding, helical distortion and cleavage, compared to a canonical LAGLIDADG homing endonuclease
Mycobacterium leprae, which has undergone reductive evolution leaving behind a minimal set of essential genes, has retained intervening sequences in four of its genes implicating a vital role for them in the survival of the leprosy bacillus. A single inframe intervening sequence has been found embedded within its recA gene. Comparison of M. leprae recA intervening sequence with the known interv...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2004
ISSN: 1362-4962
DOI: 10.1093/nar/gkh520